Basic Electrophysiological Methods

Description

• Wide-ranging coverage. Although not every conceivable technique or variation thereof is covered, much of the information on the selected methods can be generalized.
• Minimal coverage of basic neuroscience and theory allows the reader to focus on practical information.
• Accessible style covers methodological basics thoroughly, from the ground up, without being condescending.
• Liberal use of illustrations covers every aspect of a method from theory through implementation and data analysis.

This book is a concise, easy-to read guide to a selection of the most important contemporary electrophysiological techniques, their implementation, applications, and ways in which they can be combined and integrated with related neuroscience techniques. It is intended for students, postdocs and faculty with a basic neuroscience background, so does not obscure the relevant technical details with textbook neuroscience tutorials as many other books do. Each chapter provides a concise overview of the underlying theory, a comprehensive description of equipment, materials, methods, data management and analysis, a troubleshooting guide, and a list of frequently asked questions. No book or online resource can function as a strictly DIY set of instructions on how to implement a complex technique. However, this book provides a fundamental and accessible set of information intended to form a foundation prior to, during, and after hands-on experience and training, greatly facilitating the initial learning process and subsequent fine-tuning of technical details.

Readership: The potential audience is extremely broad, ranging from undergraduate and graduate students through postdocs and faculty in every branch of neuroscience.

Table of Contents

Introduction
Matt Carter and Ellen Covey

Chapter 1: Patch Clamp Recording in Brain Slices
Luke Campagnola and Paul Manis

Chapter 2: Patch Clamp Recording in vivo
David Ferster

Chapter 3: Extracellular Single Unit Recordings and Neuropharmacological Methods
William L. Coleman and R. Michael Burger

Chapter 4: Multi-electrode Recording of Neural Activity in Awake Behaving Animals
Samantha Summerson and Caleb Kemere

Chapter 5: Fast-Scan Cyclic Voltammetry in Behaving Animals
Monica M. Arnold, Lauren M. Burgeno, and Paul E. M. Phillips

Chapter 6: Electroencephalogram Recording in Humans
Julia W. Y. Kam and Todd C. Handy

Chapter 7: Electroencephalography and Local Field Potentials in Animals
Sean Reed, Sonia Jego, and Antoine Adamantidis

Chapter 8: Voltage-Sensitive Dye Imaging
William Frost and Jian-young Wu

Chapter 9: Optogenetics and Electrophysiology
Hua-an Tseng, Richie E. Kohman, and Xue Han

Glossary

Authors

Ellen Covey, PhD, University of Washington, and Edited by Matt Carter, PhD, Williams College
Ellen Covey, PhD Department of Psychology University of Washington Seattle, WA
Matt Carter, PhD Department of Biochemistry University of Washington Seattle, WA