Fe-S Cluster Enzymes. Part a (Methods in Enzymology, Vol. 595)

Description

Fe-S Cluster Enzymes, Part A, Volume 595 is the first of two volumes focused on Fe-S cluster enzymes. New topics of note in this series include Electrochemistry of Fe/S Proteins, Genetic, biochemical and biophysical methods for studying Fe-S proteins and their assembly, Fluorescent reporters to track Fe-S cluster assembly and transfer reactions, Mechanism-based strategies for structural characterization of radical SAM reaction intermediates, Purification and Characterization of Recalcitrant Cobalamin-Dependent Radical S-adenosylmethionine Methylases, A polymerase with potential: the Fe-S cluster in Human DNA Primase, In Vitro Studies of Cellular Iron-sulfur Cluster Biosynthesis, Trafficking and Transport, and Fe-S cluster Hsp70 Chaperones: the ATPase cycle and protein interactions.

• Contain contributions from leading authorities on enzymology
• Informs and updates on all the latest developments in the field

Table of Contents

1. De Novo Design of Iron–Sulfur Proteins
Zahra B. Dizicheh, Nicholas Halloran, William Asma and Giovanna Ghirlanda

2. In Vitro Studies of Cellular Iron–Sulfur Cluster Biosynthesis, Trafficking, and Transport
Christine Wachnowsky and J.A. Cowan

3. A Polymerase With Potential: The Fe–S Cluster in Human DNA Primase
Marilyn E. Holt, Lauren E. Salay and Walter J. Chazin

4. Combined Biochemical, Biophysical, and Cellular Methods to Study Fe–S Cluster Transfer and Cytosolic Aconitase Repair by MitoNEET
Cécile Mons, Ioana Ferecatu, Sylvie Riquier, Ewen Lescop, Cécile Bouton and Marie-Pierre Golinelli-Cohen

5. Defining the Architecture of the Core Machinery for the Assembly of Fe–S Clusters in Human Mitochondria  
O. Gakh, W. Ranatunga, B.K. Galeano, D.S. Smith IV, J.R. Thompson and G. Isaya

6. Fe–S Cluster Hsp70 Chaperones: The Atpase Cycle and Protein Interactions
Rafal Dutkiewicz, Malgorzata Nowak, Elizabeth A. Craig and Jaroslaw Marszalek

7. B. subtilis as a Model for Studying the Assembly of Fe–S Clusters in Gram-Positive Bacteria
Patricia C. Dos Santos

8. Genetic, Biochemical, and Biophysical Methods for Studying Fe–S Proteins and Their Assembly
S. Ollagnier-de Choudens and F. Barras

9. Structural Characterization of Poised States in the Oxygen Sensitive Hydrogenases and Nitrogenases
Jacob H. Artz, Oleg A. Zadvornyy, David W. Mulder, Paul W. King and John W. Peters

10. Nitrogenase Assembly: Strategies and Procedures
Nathaniel S. Sickerman, Yilin Hu and Markus W. Ribbe

11. TsrM as a Model for Purifying and Characterizing Cobalamin-Dependent Radical S-Adenosylmethionine Methylases
Anthony J. Blaszczyk, Roy X. Wang and Squire J. Booker

12. Mechanism-Based Strategies for Structural Characterization of Radical SAM Reaction Intermediates
K.M. Davis and A.K. Boal