Analytical Ultracentrifugation, the latest volume in Methods in Enzymology, focuses on analytical ultracentrifugation. The scope of this technique has greatly expanded in recent years due to advances in instrumentation, algorithms and software.
This volume describes the latest innovations in the field and in the applications
of analytical ultracentrifugation in the analysis of macromolecules, macromolecular
assemblies, and biopharmaceuticals.
- ITimely contribution that describes a rapidly changing field
- Leading researchers in the field
- Broad coverage: instrumentation, basic theory, data analysis, and applications
The intended audience includes: biophysicists interested in macromolecular interactions, thermodynamics and hydrodynamics, biochemists and molecular biologists who wish to use analytical ultracentrifugation, and researchers in the biotechnology industry who are interested in the applications of analytical ultracentrifugation in characterization of biopharmaceuticals.
- Next Generation AUC adds a Spectral Dimension: Development of Multiwavelength
Detectors for the Analytical Ultracentrifuge
Joseph Pearson, Frank Krause, Dirk Haffke, Borries Demeler, Kristian Schilling and Helmut Cölfen
- Next Generation AUC: Analysis of Multi-Wavelength Analytical Ultracentrifugation
Gary E. Gorbet, Joseph Z. Pearson, Aysha K. Demeler, Helmut Cölfen and Borries Demeler
- Sedimentation Velocity: A Classical Perspective
John J. Correia and Walter F. Stafford
- Hydrodynamic Modeling and its Application in AUC
Mattia Rocco and Olwyn Byron
- Calculations and Publication-Quality Illustrations for Analytical Ultracentrifugation
Chad A. Brautigam
- Sedimentation Equilibrium Analysis of Clpb Self-Association in Diluted
and Crowded Solutions.
Carlos Alfonso, Urko del Castillo, Ianire Martín, Arturo Muga and Germán Rivas
- Analysis of Linked Equilibria
JiaBei Lin and Aaron L. Lucius
- Elucidating Complicated Assembling Systems in Biology Using Size-And-Shape
Analysis of Sedimentation Velocity Data
Catherine T. Chaton and Andrew B. Herr
- Quaternary Structure Analyses of an Essential Oligomeric Enzyme
Tatiana P. Soares da Costa, Janni B. Christensen, Sebastien Desbois, Shane E. Gordon, Ruchi Gupta, Campbell J. Hogan, Tao G. Nelson, Matthew T. Downton, Chamodi K. Gardhi, Belinda M. Abbott, John Wagner, Santosh Panjikar and Matthew A. Perugini
- Characterization of Intrinsically Disordered Proteins by Analytical Ultracentrifugation
David J. Scott and Donald J. Winzor
- Sedimentation Velocity Analysis of the Size Distribution of Amyloid Oligomers
Yee-Foong Mok, Geoffrey J. Howlett and Michael D.W. Griffin
- AUC and Small Angle Scattering for Membrane Proteins
Aline Le Roy, Kai Wang, Béatrice Schaack, Peter Schuck, Cécile Breyton and Christine Ebel
- Hydrodynamic Models of G-quadruplex Structures
Jonathan B. Chaires, William L. Dean, Huy Le and John O. Trent
- Analytical Ultracentrifugation as a Tool to Study Non-specific Protein-DNA
Teng-Chieh Yang, Carlos Enrique Catalano and Nasib Karl Maluf
- Characterization of Homogeneous, Cooperative Protein-DNA Clusters by Sedimentation
Equilibrium Analytical Ultracentrifugation and Atomic Force Microscopy
Ingrid Tessmer and Michael G. Fried
- Sedimentation Velocity Analysis of Large Oligomeric Chromatin Complexes
Using Interference Detection.
Hansen and Jeffrey
- Methods in Enzymology - Analytical Ultracentrifugation
David L Bain, Rolando W De Angelis, Keith D Connaghan, Qin Yang, Gregory D Degala and James R Lambert
- Ultracentrifuge Methods for the Analysis of Polysaccharides, Glycoconjugates
Stephen E. Harding, Gary G. Adams, Fahad Almutairi, Qushmua Alzahrani, Tayyibe Erten, M. Samil Kök and Richard B. Gillis
- Analytical Ultracentrifugation and its Role in Development and Research
of Therapeutical Proteins.
Jun Liu, Sandeep Yadav, James Andya, Barthélemy Demeule and Steven J. Shire
- Guidance to Achieve Accurate Aggregate Quantitation in Biopharmaceuticals
Kelly K. Arthur, Brent S. Kendrick and John P. Gabrielson
- Protein Assembly in Serum and the Differences from Assembly in Buffer.
John J. Hill and Thomas M. Laue
Dr. Cole received a Sc.B. in Biochemistry from Brown University. He obtained his Ph.D. in Biophysical Chemistry at UC Berkeley where he investigated the mechanism of photosynthetic oxygen evolution. He carried out postdoctoral research at Stanford University on the structure and function of metalloenzymes that interact with oxygen. In 1991, he took a position at Merck Research Laboratories in antiviral drug discovery. Dr. Cole applied analytical ultracentrifugation and related biophysical techniques to probe macromolecular interactions that regulate replication of HIV, Influenza and Hepatitis C virus. In 2001, he to return to academia and moved to the Department of Molecular and Cell Biology at the University of Connecticut, where he is professor of structural biology, biophysics and biochemistry. His research group uses biophysical approaches to characterize macromolecular interactions that regulate important biological processes. A major focus is the innate immunity pathway for defense against viral infection. In an NIH-supported research program, his group has defined the mechanism for activation of protein kinase R by RNA. He directs the National Analytical Ultracentrifugation Facility and holds a joint appointment in the department of Chemistry at the University of Connecticut. He is a member of the editorial board of Biophysical Journal. He is the author of 84 publications and reviews.