Electron Paramagnetic Resonance Investigations of Biological Systems…. Part B (Methods in Enzymology, Vol. 564)

Description

Electron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions Part A & B, are the latest volumes in the Methods in Enzymology series, continuing the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods centered on the use of Electron Paramagnetic Resonance (EPR) techniques to study biological structure and function.

Key Features

• Timely contribution that describes a rapidly changing field
• Leading researchers in the field
• Broad coverage: Instrumentation, basic theory, data analysis, and applications

Readership: Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.

Contents

Section 1. Spin Labeling Studies of Proteins
1. Saturation Recovery EPR and Nitroxide Spin Labeling for Exploring Structure and Dynamics in Proteins
Zhongyu Yang, Michael Bridges, Michael Lerch, Christian Altenbach and Wayne L. Hubbell
2. High-Pressure Epr and Site-Directed Spin Labeling for Mapping Molecular Flexibility in Proteins
Michael T. Lerch, Zhongyu Yang, Christian Altenbach and Wayne L. Hubbell
3. Exploring Structure, Dynamics, and Topology of Nitroxide Spin-Labeled Proteins Using Continuous-Wave Electron Paramagnetic Resonance Spectroscopy
Christian Altenbach, Carlos J. López, Kálmán Hideg, and Wayne L. Hubbell
4. Bifunctional Spin Labeling of Muscle Proteins: Accurate Rotational Dynamics, Orientation, and distance by EPR
Andrew R. Thompson, Benjamin P. Binder, Jesse E. McCaffrey, Bengt Svensson, and David D. Thomas
5. EPR Distance Measurements in Deuterated Proteins
Hassane El Mkami and David G. Norman
6. Spin-Labeling Studies of HIV Protease
Thomas M. Casey and Gail E. Fanucci

Section 2. Spin Labeling Studies of Membrane and Membrane-Associated Proteins
7. Ionizable Nitroxides for Studying Local Electrostatic Properties of Lipid Bilayers and Protein Systems by EPR
Maxim A. Voinov and Alex I. Smirno
8. Peptide-Membrane Interactions by Spin-labeling EPR
Tatyana I. Smirnova and Alex I. Smirnov
9. Structural Characterization of Membrane Curving Proteins: Site-Directed Spin-Labeling, EPR, and Computational Refinement
Mark R. Ambroso, Ian S. Haworth and Ralf Langen
10. Determining the Secondary Structure of Membrane Proteins and Peptides via Electron Spin Echo Envelope Modulation (ESEEM) Spectroscopy
Lishan Liu, Daniel J. Mayo, Indra D. Sahu, Andy Zhou, Rongfu Zhang, Robert M. McCarrick and Gary A. Lorigan
11. Spin Labeling Studies of Transmembrane Signaling and Transport: Applications to Photo-/Chemotaxis, ABC Transporters and Symporters
Johann P. Klare and Heinz-Jürgen Steinhoff
12. Navigating Membrane Protein Structure, Dynamics, and Energy Landscapes using Spin Labeling and EPR Spectroscopy
Derek P Claxton, Kelli Kazmier, Smriti Mishra and Hassane S Mchaourab
13. Spin Labeling of Potassium Channels
Dylan Burdette and Adrian Gross

Section 3. Spin Labeling Studies of Nucleic Acids
14. Advanced EPR Methods for Studying Conformational Dynamics of Nucleic Acids
B. Endeward, A. Marko, V. P. Denysenkov, S. Th. Sigurdsson, T. F. Prisner
15. An Integrated Spin-Labeling/Computational-Modeling Approach for Mapping Global Structures of Nucleic Acids
Narin S. Tangprasertchai, Xiaojun Zhang, Yuan Ding, Kenneth Tham,Remo Rohs Ian S. Haworth, and Peter Z. Qin

Section 4. EPR-NMR Methods and Applications
16. Overhauser Dynamic Nuclear Polarization Studies on Local Water Dynamics
Ilia Kaminker, Ryan Barnes and Songi Han
17. Practical Aspects of Paramagnetic Relaxation Enhancement in Biological Macromolecules
G. Marius Clore

Section 5. In Vivo EPR Oxymetry and Imaging
18. In Vivo pO2 imaging of tumors: Oxymetry with Very Low Frequency Electron Paramagnetic Resonance
Boris Epel and Howard J. Halpern
19. Direct and Repeated Measurement of Heart and Brain Oxygenation Using in Vivo EPR Oximetry
Nadeem Khan, Huagang Hou, Harold M. Swartz, and Periannan Kuppusamy
20. Free Radical Imaging using in vivo Dynamic Nuclear Polarization-MRI
Hideo Utsumi and Fuminori Hyodo