Electron Paramagnetic Resonance Investigations of Biological Systems…. Part a (Methods in Enzymology, Vol. 563)

Description

Electron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions Part A & B, are the latest volumes in the Methods in Enzymology series, continuing the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods centered on the use of Electron Paramagnetic Resonance (EPR) techniques to study biological structure and function.

Key Features

• Timely contribution that describes a rapidly changing field
• Leading researchers in the field
• Broad coverage: Instrumentation, basic theory, data analysis, and applications

Readership: Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.

Contents

Section. 1 EPR Methodology and Instrumentation
1. Rapid-Scan Epr of Nitroxide Spin Labels and Semiquinones
Sandra S. Eaton and Gareth R. Eaton
2. Fourier Deconvolution Methods for Resolution Enhancement in Continuous-Wave EPR spectroscopy
George H. Reed and Russell R. Poyner
3. Multifrequency Pulsed EPR and the Characterization of Molecular Dynamics
Sandra S. Eaton and Gareth R. Eaton
4. Resolution and Characterization of Chemical Steps in Enzyme Catalytic Sequences by Using Low Temperature and Time-Resolved, Full-Spectrum EPR Spectroscopy in Fluid Cryosolvent and Frozen Solution Systems
Miao Wang, Chen Zhu, Meghan M. Kohne and Kurt Warncke
5. 230/115 Ghz Electron Paramagnetic Resonance/Double Electron-Electron Resonance Spectroscopy
Franklin H. Cho, Viktor Stepanov, Chathuranga Abeywardana, and Susumu Takahashi

Section 2. EPR Spectral Simulation and Software
6. CW-EPR Spectral Simulations: Solid State
Stefan Stoll
7. CW-EPR Spectral Simulations: Slow-Motion Regime
David E. Budil
8. Quantitative Interpretation of Multifrequency Multimode EPR Spectra of Metal Containing Proteins, Enzymes and Biomimetic Complexes.
Doros T. Petasis and Michael P. Hendrich

Section 3. EPR Studies of Metal-Containing Proteins and Metal Cofactors
9. Pulse Double-Resonance EPR Techniques for the Study of Metallobiomolecules
Nicholas Cox, Anna Nalepa, Maria-Eirini Pandelia Wolfgang Lubitz, Anton Savitsky
10. Mapping the Structure of Metalloproteins with RIDME
Andrei V. Astashkin
11. Structural Characterization of the Catalytic Sites of Mononuclear Non-Heme Fe Hydroxylases using 2H-ESEEM
John McCracken
12. Pulsed EPR in the Study of Drug Binding in Cytochrome P450 and NOS
Alex A. Cruce, Molly Lockart and Michael K. Bowman
13. EPR Methods for Biological Cu(II): L-Band CW and NARS
Brian Bennett and Jason Kowalski

Section 4. Advances in Spin Label Incorporation
14. Development and Application of Spin Traps, Spin Probes and Spin Labels
Elena G. Bagryanskaya, Olesya A. Krumkacheva, Matvey V. Fedin and Sylvain R. A. Marque
15. Site-Directed Spin Labeling of RNA by Post-Synthetic Modification of 2'-Amino Groups
Subham Saha, Anil P. Jagtap and Snorri Th. Sigurdsson
16. Gd3+ Spin Labeling for Measuring Distances in Diomacromolecules: why and how?
Akiva Feintuch, Gottfried Otting, and Daniella Goldfarb
17. Cu2+ as an ESR Probe of Protein Structure and Function
Zhongyu Yang, Ming Ji, Timothy F. Cunningham, and Sunil Saxena
18. Genetically Encoded Spin Labels for In Vitro and In Cell EPR Studies of Native Proteins
M. J. Schmidt, A. Fedoseev, D. Summerer, and M. Drescher
19. Genetic Incorporation of the Unnatural Amino Acid p-Acetyl Phenylalanine into Proteins for Site-Directed Spin Labeling
Eric G. B. Evans and Glenn L. Millhauser

Section 5. Inter-Spin Label Distance Measurements: Analysis Considerations and Approaches
20. A Straightforward Approach to the Analysis of Double Electron-Electron Resonance Data
Richard A. Stein, Albert H. Beth and Eric J. Hustedt
21. Computer Modeling of Spin Labels: NASNOX, PRONOX and ALLNOX
Kathleen N. Beasley, Brian T. Sutch, Ma'mon M. Hatmal, Ralf Langen, Peter Z. Qin and Ian S. Haworth
22. Mtsslsuite: Probing Biomolecular Conformation y Spin Labeling Studies
Gregor Hagelueken, Dinar Abdullin and Olav Schiemann
23. Simulation of Spin Label Structure and its Implication in Molecular Characterization
Piotr Fajer, Mikolai Fajer, Michael Zawrotny and Wei Yang