RNA Modification (Methods in Enzymology, Vol. 560)

Description

RNA Modification provides a useful examination of the science and its role in biological regulation, the current frontier of life science research, and includes various RNA modications and their role in gene expression. It represents the most up-to-date knowledge and protocols available today.

Key Features

• Dynamic RNA modifications and their roles in biological regulation are the current frontier of life science research
• This volume of Methods in Enzymology represents up to date knowledge and protocols

Readership: Scientists interested in RNA modifications, post-transcriptional gene expression regulation and regulatory RNA.

Contents

1. Methodology For The High-Throughput Identification and Characterization of tRNA Variants that are Substrates for a tRNA Decay Pathway
   Matthew J. Payea, Michael P. Guy, and Eric M. Phizicky
2. Nucleoside Analysis by Hydrophilic Interaction Liquid Chromatography Coupled with Mass Spectrometry
   Yuriko Sakaguchi, Kenjyo Miyauchi, Byeong-il Kang, and Tsutomu Suzuki
3. A Platform for Discovery and Quantification of Modified Ribonucleosides in RNA: Application to Stress-Induced Reprogramming of tRNA Modifications
   Weiling Maggie Cai, Yok Hian Chionh, Fabian Hia, Chen Gu, Stefanie Kellner, Megan E. McBee, Chee Sheng Ng, Yan Ling Joy Pang, Erin G. Prestwich, Kok Seong Lim, I. Ramesh Babu, Thomas J. Begley and Peter C. Dedon
4. Recognition of Specified RNA Modifications by the Innate Immune System
   Tatjana Eigenbrod, Patrick Keller, Steffen Kaiser, Katharina Rimbach, Alexander H. Dalpke and Mark Helm
5. Kinetic Analysis of tRNA Methyl Transferases
   Ya-Ming Hou and Isao Masuda
6. Preparation of Human Nuclear RNA m6A Methyltransferases and Demethylases and Biochemical Characterization of Their Catalytic Activity
   Jianzhao Liu, Yanan Yue, and Chuan He
7. Transcriptome-Wide Mapping of N6-Methyladenosine by m6A-Seq
   Dan Dominissini, Sharon Moshitch Moshkovitz, Ninette Amariglio and Gideon Rechavi
8. Probing RNA Modification Status at Single-Nucleotide Resolution in Total RNA
   Nian Liu and Tao Pan
9. High-resolution mapping of N6-methyladenosine in transcriptome and Genome Using a Photo-Crosslinking-Assisted Strategy
   Kai Chen, Guan-Zheng Luo and Chuan He
10. Pseudouridine in Mrna: Incorporation, Detection and Recoding
    Guowei Wu, Chao Huang and Yi-Tao Yu
11. Pseudo-Seq: Genome-Wide Detection of Pseudouridine Modifications in RNA
    T homas M. Carlile, Maria F. Rojas-Duran and Wendy V. Gilbert
12. Pseudouridine Chemical Labeling and Profiling
    Xiaoyu Li, Shiqing Ma and Chengqi Yi
13. Experimental Approaches for Target Profiling RNA Cytosine Methyltransferases
    Vahid Khoddami, Archana Yerra, and Bradley R. Cairns
14. RNA 5-Methylcytosine Analysis by Bisulfite Sequencing
    Matthias Schaefer
15. Biochemical and transcriptome-wide identification of A-to-I RNA editing sites by ICE-seq
    Shunpei Okada, Masayuki Sakurai, Hiroki Ueda, and Tsutomu Suzuki
16. Radical SAM-Mediated Methylation of Ribosomal RNA
    Vanja Stojkovic and Danica Galonic Fujimori

Author

Chuan He, Ph.D., is the John T. Wilson Distinguished Service Professor in the Department of Chemistry and Director of the Institute for Biophysical Dynamics at the University of Chicago. He is also a joint Professor in the Department of Chemical Biology and Director of the Synthetic and Functional Biomolecules Center at Peking University. He was born in P. R. China in 1972 and received his B.S. (1994) from the University of Science and Technology of China. He received his Ph. D. degree from Massachusetts Institute of Technology in chemistry in 2000. After being trained as a Damon-Runyon postdoctoral fellow at Harvard University from 2000-2002, he joined the University of Chicago as an Assistant Professor, and was promoted to Associate Professor in 2008, Professor in 2010 and John T. Wilson Distinguished Service Professor in 2014. He is also a member of the Cancer Research Center at the University of Chicago. His research spans a broad range of chemical biology, epigenetics, cell biology, molecular biology, biochemistry, structural biology, and genomics. His recent research concerns reversible RNA and DNA methylation in biological regulation. His research group discovered the first RNA demethylase and showed that reversible RNA methylation significantly affects post-transcriptional gene expression regulation. He was selected as an Investigator of the Howard Hughes Medical Institute in 2013.