Membrane Proteins - Engineering, Purification and Crystallization (Methods in Enzymology, Vol. 557)

Description

Membrane Proteins – Engineering, Purification and Crystallization, a volume of Methods In Enzymology, encompasses chapters from the leading experts in the area of membrane protein biology. The chapters provide a brief overview of the topics covered and also outline step-by-step protocol for the interested audience. Illustrations and case example images are included wherever appropriate to help the readers understand the schematics and general experimental outlines.

• Volume of Methods In Enzymology
• Contains a collection of a diverse array of topics in the area of membrane protein biology ranging from recombinant expression, isolation, functional characterization, biophysical studies and crystallization

Contents

Section I. Membrane Protein Engineering, Solubilization and Purification

• 1.Multicolor fluorescence-based screening toward structural analysis of multiprotein membrane complexes
  Simon Trowitzsch & Robert Tampé
• 2.A novel screening approach for optimal and functional fusion of T4 lysozyme in GPCRs
  Elizabeth Mathew and Mark E Dumont
• 3.Membrane preparation and solubilization
  Ankita Roy
• 4.Amphipathic agents for membrane protein study
  Aiman Sadaf, Kyung Ho Cho, Bernadette Byrne, Pil Seok Chae
• 5.Quantification of detergent using colorimetric methods in membrane protein crystallography
  Chelsy Prince , Zongchao Jia
• 6.Solubilization of G Protein-Coupled Receptors: A Convenient Strategy to Explore Lipid-Receptor Interaction
  Amitabha Chattopadhyay, Bhagyashree D. Rao and Md. Jafurulla
• 7.Overexpression, Isolation, Purification and Crystallization of NhaA
  Etana Padan and Manish Dwivedi
• 8.Purification, refolding and crystallization of the outer membrane protein OmpG from Escherichia coli
  Stefan Köster, Katharina van Pee, Özkan Yildiz
• 9.Biophysical Approaches to the Study of LeuT, a Prokaryotic Homolog of Neurotransmitter Sodium Symporters
  Satinder K. Singh and Aritra Pal

Section II. Generation and Use of Antibody Fragments Against Membrane Proteins

• 10.Generation of recombinant antibody fragments for membrane protein crystallization
  Syed H. Mir , Claudia Escher, Wei-Chun Kao, Dominic Birth, Christophe Wirth, Carola Hunte
• 11.Phage Display Selections for Affinity Reagents to Membrane Proteins in Nanodiscs
  Pawel K. Dominik, and Anthony A. Kossiakoff
• 12.Antibody fragments for crystallization, trapping of active conformations and stabilization of G-protein coupled receptors and their signaling complexes
  Arun K. Shukla, Charu Gupta, Ashish Srivastava and Deepika Jaiman

Section III. Biophysical Studies of Membrane Proteins

• 13.Conformational Analysis of G Protein-Coupled Receptor Signaling by Hydrogen/Deuterium Exchange Mass Spectrometry
  Sheng Li, Su Youn Lee and Ka Young Chung
• 14.EPR Studies of Gating Mechanisms in Ion Channels
  Sudha Chakrapani
• 15.Magic-Angle-Spinning Solid-State NMR of Membrane Proteins
  Lindsay A Baker, Gert E Folkers, Tessa Sinnige, Klaartje Houben, Mohammed Kaplan, Elwin AJ van der Cruijsen, Marc Baldus
• 16.Solution NMR Structure Determination of Polytopic a-helical membrane proteins: A guide to spin label paramagnetic relaxation enhancement restraints
  Linda Columbus and Brett Kroncke

Section IV. Crystallization of Membrane Proteins

• 17.Inducing Two-Dimensional Crystallization of Membrane Proteins by Dialysis for Electron Crystallography
  Yusuf M. Uddin and Ingeborg Schmidt-Krey
• 18.Crystallization of Membrane Proteins by Vapor Diffusion
  Jared A. Delmar, Jani Reddy Bolla, Chih-Chia Su, and Edward W. Yu
• 19.An empirical approach to bicelle crystallization
  Sandra Poulos, Jacob L. W. Morgan, Jochen Zimmer, Salem Faham
• 20.Fluorescence Recovery After Photobleaching in Lipidic Cubic Phase (LCP-FRAP): A pre-crystallization assay for membrane proteins
  Gustavo Fenalti, Enrique E. Abola, Chong Wang, Beili Wu, Vadim Cherezov
• 21.Crystallization of Proteins from Crude Bovine Rod Outer Segments
  Bo Y Baker, Sahil Gulati, Wuxian Shi, Benlian Wang, Phoebe L. Stewart, and Krzysztof Palczewski
• 22.Crystallization of Photosystem II for Time Resolved Structural Studies Using an X-ray Free Electron Laser
  Jesse Coe, Christopher Kupitz, Shibom Basu, Chelsie E. Conrad, Shatabdi Roy-Chowdhury, Raimund Fromme, Petra Fromme

Section V. Computational Approaches to Understand Membrane Proteins

• 23.Major Intrinsic Protein Superfamily: Channels with Unique Structural Features and Diverse Selectivity Filters
  Ravi Kumar Verma, Anjali Bansal Gupta and Ramasubbu Sankararamakrishnan
• 24.Comparative Sequence-Function Analysis of the Major Facilitator Superfamily: the ‘Mix-and-Match’ Method
  M. Gregor Madej
• 25.Elucidating Ligand-Modulated Conformational Landscape of GPCRs Using Cloud-computing Approaches
  Diwakar Shukla, Morgan Lawrenz and Vijay S. Pande

Author

Dr. Arun K. Shukla obtained his M.Sc. (Master in Science) from the Center for Biotechnology at the Jawaharlal Nehru University in New Delhi, India. Dr. Shukla did his Ph.D. from the Department of Molecular Membrane Biology at the Max Planck Institute of Biophysics in Frankfurt, Germany. His Ph.D. research work was focused on structural studies of G Protein-Coupled Receptors (GPCRs).

Dr. Shukla subsequently carried out his post-doctoral work in the Department of Medicine at the Duke University in North Carolina, USA. During his post-doctoral research work, Dr. Shukla focused on understanding the biophysical and structural basis of ß-arrestin mediated regulation of GPCRs and non-canonical GPCR signaling. Dr. Shukla has served as an Assistant Professor in the Department of Medicine at the Duke University in Durham, North Carolina, USA.

Dr. Shukla is currently an Assistant Professor in Department of Biological Sciences and Bioengineering at the Indian Institute of Technology, Kanpur, India. Dr. Shukla is also an Intermediate Fellow of the Wellcome Trust-DBT India Alliance. The research program in Dr. Shukla’s laboratory is focused on understanding the molecular mechanism of activation, signaling and regulation of G Protein-Coupled Receptors.