


Membrane Proteins - Engineering, Purification and Crystallization (Methods in Enzymology, Vol. 557)
Shukla, A.
1ª Edición Mayo 2015
Inglés
Tapa dura
644 pags
1092 gr
16 x 24 x 3 cm
ISBN 9780128021835
Editorial ACADEMIC PRESS
Recíbelo en un plazo De 7 a 10 días
Description
Membrane Proteins – Engineering, Purification and Crystallization, a volume of Methods In Enzymology, encompasses chapters from the leading experts in the area of membrane protein biology. The chapters provide a brief overview of the topics covered and also outline step-by-step protocol for the interested audience. Illustrations and case example images are included wherever appropriate to help the readers understand the schematics and general experimental outlines.
- Volume of Methods In Enzymology
- Contains a collection of a diverse array of topics in the area of membrane protein biology ranging from recombinant expression, isolation, functional characterization, biophysical studies and crystallization
Contents
Section I. Membrane Protein Engineering, Solubilization and Purification
- 1.Multicolor fluorescence-based screening toward structural analysis of
multiprotein membrane complexes
Simon Trowitzsch & Robert Tampé - 2.A novel screening approach for optimal and functional fusion of T4 lysozyme
in GPCRs
Elizabeth Mathew and Mark E Dumont - 3.Membrane preparation and solubilization
Ankita Roy - 4.Amphipathic agents for membrane protein study
Aiman Sadaf, Kyung Ho Cho, Bernadette Byrne, Pil Seok Chae - 5.Quantification of detergent using colorimetric methods in membrane protein
crystallography
Chelsy Prince , Zongchao Jia - 6.Solubilization of G Protein-Coupled Receptors: A Convenient Strategy to
Explore Lipid-Receptor Interaction
Amitabha Chattopadhyay, Bhagyashree D. Rao and Md. Jafurulla - 7.Overexpression, Isolation, Purification and Crystallization of NhaA
Etana Padan and Manish Dwivedi - 8.Purification, refolding and crystallization of the outer membrane protein
OmpG from Escherichia coli
Stefan Köster, Katharina van Pee, Özkan Yildiz - 9.Biophysical Approaches to the Study of LeuT, a Prokaryotic Homolog of
Neurotransmitter Sodium Symporters
Satinder K. Singh and Aritra Pal
Section II. Generation and Use of Antibody Fragments Against Membrane Proteins
- 10.Generation of recombinant antibody fragments for membrane protein crystallization
Syed H. Mir , Claudia Escher, Wei-Chun Kao, Dominic Birth, Christophe Wirth, Carola Hunte - 11.Phage Display Selections for Affinity Reagents to Membrane Proteins in
Nanodiscs
Pawel K. Dominik, and Anthony A. Kossiakoff - 12.Antibody fragments for crystallization, trapping of active conformations
and stabilization of G-protein coupled receptors and their signaling complexes
Arun K. Shukla, Charu Gupta, Ashish Srivastava and Deepika Jaiman
Section III. Biophysical Studies of Membrane Proteins
- 13.Conformational Analysis of G Protein-Coupled Receptor Signaling by Hydrogen/Deuterium
Exchange Mass Spectrometry
Sheng Li, Su Youn Lee and Ka Young Chung - 14.EPR Studies of Gating Mechanisms in Ion Channels
Sudha Chakrapani - 15.Magic-Angle-Spinning Solid-State NMR of Membrane Proteins
Lindsay A Baker, Gert E Folkers, Tessa Sinnige, Klaartje Houben, Mohammed Kaplan, Elwin AJ van der Cruijsen, Marc Baldus - 16.Solution NMR Structure Determination of Polytopic a-helical membrane
proteins: A guide to spin label paramagnetic relaxation enhancement restraints
Linda Columbus and Brett Kroncke
Section IV. Crystallization of Membrane Proteins
- 17.Inducing Two-Dimensional Crystallization of Membrane Proteins by Dialysis
for Electron Crystallography
Yusuf M. Uddin and Ingeborg Schmidt-Krey - 18.Crystallization of Membrane Proteins by Vapor Diffusion
Jared A. Delmar, Jani Reddy Bolla, Chih-Chia Su, and Edward W. Yu - 19.An empirical approach to bicelle crystallization
Sandra Poulos, Jacob L. W. Morgan, Jochen Zimmer, Salem Faham - 20.Fluorescence Recovery After Photobleaching in Lipidic Cubic Phase (LCP-FRAP):
A pre-crystallization assay for membrane proteins
Gustavo Fenalti, Enrique E. Abola, Chong Wang, Beili Wu, Vadim Cherezov - 21.Crystallization of Proteins from Crude Bovine Rod Outer Segments
Bo Y Baker, Sahil Gulati, Wuxian Shi, Benlian Wang, Phoebe L. Stewart, and Krzysztof Palczewski - 22.Crystallization of Photosystem II for Time Resolved Structural Studies
Using an X-ray Free Electron Laser
Jesse Coe, Christopher Kupitz, Shibom Basu, Chelsie E. Conrad, Shatabdi Roy-Chowdhury, Raimund Fromme, Petra Fromme
Section V. Computational Approaches to Understand Membrane Proteins
- 23.Major Intrinsic Protein Superfamily: Channels with Unique Structural
Features and Diverse Selectivity Filters
Ravi Kumar Verma, Anjali Bansal Gupta and Ramasubbu Sankararamakrishnan - 24.Comparative Sequence-Function Analysis of the Major Facilitator Superfamily:
the ‘Mix-and-Match’ Method
M. Gregor Madej - 25.Elucidating Ligand-Modulated Conformational Landscape of GPCRs Using
Cloud-computing Approaches
Diwakar Shukla, Morgan Lawrenz and Vijay S. Pande
Author
Dr. Arun K. Shukla obtained his M.Sc. (Master in Science) from the Center for Biotechnology at the Jawaharlal Nehru University in New Delhi, India. Dr. Shukla did his Ph.D. from the Department of Molecular Membrane Biology at the Max Planck Institute of Biophysics in Frankfurt, Germany. His Ph.D. research work was focused on structural studies of G Protein-Coupled Receptors (GPCRs).
Dr. Shukla subsequently carried out his post-doctoral work in the Department of Medicine at the Duke University in North Carolina, USA. During his post-doctoral research work, Dr. Shukla focused on understanding the biophysical and structural basis of ß-arrestin mediated regulation of GPCRs and non-canonical GPCR signaling. Dr. Shukla has served as an Assistant Professor in the Department of Medicine at the Duke University in Durham, North Carolina, USA.
Dr. Shukla is currently an Assistant Professor in Department of Biological Sciences and Bioengineering at the Indian Institute of Technology, Kanpur, India. Dr. Shukla is also an Intermediate Fellow of the Wellcome Trust-DBT India Alliance. The research program in Dr. Shukla’s laboratory is focused on understanding the molecular mechanism of activation, signaling and regulation of G Protein-Coupled Receptors.
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