Methods of Adipose Tissue Biology, Part B (Methods in Enzymology, Vol. 538)

Table of Contents

1. Preparation and Differentiation of Mesenchymal Stem Cells from Ears of Adult Mice
   Barbara Gawronska-Kozak
2. 3-D Adipocyte Differentiation and Peri-Adipocyte Collagen Turnover
   Tae-Hwa Chun and Mayumi Inoue
3. Differentiation of White and Brown Adipocytes from Human Pluripotent Stem Cells
   Youn-Kyoung Lee and Chad A. Cowan
4. Optimal Protocol for the Differentiation and Metabolic Analysis of Human Adipose Stromal Cells
   Mi-Jeong Lee and Susan K. Fried
5. Human Adipose Derived Stromal/Stem Cell Isolation, Culture and Osteogenic Differentiation
   Ammar T. Qureshi, Cong Chen, Forum Shah, Caasy Thomas-Porch, Jeffrey M. Gimble and Daniel J. Hayes
6. Analysis of Adipose Tissue Lipid Using Mass Spectrometry
   Rodney C. Baker, Yana Nikitina and Angela R. Subauste
7. Measurement of Long-Chain Fatty Acid Uptake into Adipocytes
   Elena Dubikovskaya, Rostislav Chudnovskiy, Grigory Karateev, Hyo Min Park and Andreas Stahl
8. Measurement of the Unfolded Protein Response (UPR) to Investigate its Role in Adipogenesis and Obesity
   Jaeseok Han and Randal J. Kaufman
9. Application of Activity-Based Protein Profiling to Study Enzyme Function in Adipocytes
   Andrea Galmozzi, Eduardo Dominguez, Benjamin F. Cravatt and Enrique Saez
10. Measurement of Lipolysis
    Martina Schweiger, Thomas O. Eichmann, Ulrike Taschler, Robert Zimmermann, Rudolf Zechner and Achim Lass
11. Measurement of Lipolysis Products Secreted by 3t3-L1 Adipocytes Using Microfluidics
    Colleen E. Dugan and Robert T. Kennedy
12. Methods for Performing Lipidomics in White Adipose Tissue
    Lee D. Roberts, James A. West, Antonio Vidal-Puig and Julian L. Griffin
13. Measuring Respiratory Activity of Adipocytes and Adipose Tissues in Real Time
    Anne Bugge, Lea Dib and Sheila Collins
14. Detecting Protein Carbonylation in Adipose Tissue and in Cultured Adipocytes
    Qinghui Xu, Wendy S. Hahn and David A. Bernlohr
15. 15. Use of Fluorescence Microscopy to Probe Intracellular Lipolysis
    Emilio P. Mottillo, George M. Paul, Hsiao-Ping H. Moore and James G. Granneman
16. Evaluation of Protein Phosphorylation during Adipogenesis
    Xi Li, Rong Zeng and Qi-Qun Tang

Author Info

Ormond A Macdougald, John A. Faulkner Collegiate Professor in Physiology, and a Professor of Molecular & Integrative Physiology, and Internal Medicine, University of Michigan Medical School