Cellulases (Methods in Enzymology, Vol. 510)

Description

Cellulase refers to a class of enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze cellulolysis.This volume in the Methods in Enzymology series comprehensively covers this topic. With an international board of authors, this volume covers subjects such as "The DNSA reducing assay for measuring cellulases", "Measuring processivity" and "In situ cellulose detection with carbohydrate-binding modules".

Table of Contents

1. Measurement of endo-1,4-b-glucanase
   Barry V. McCleary, Vincent McKie and Anna Draga
2. Biomass Conversion determined via Fluorescent Cellulose Decay assay
   Bente Wischmann, Marianne Toft, Marco Malten and KC McFarland
3. The analysis of saccharification in biomass using an automated high-throughput method
   Caragh Whitehead, Leonardo D. Gomez, Simon J. McQueen-Mason
4. Studies of enzymatic cleavage of cellulose using Polysaccharide Analysis by Carbohydrate gel Electrophoresis
   (PACE)
   Ondrej Kosik, Jennifer Bromley, Marta Busse-Wicher, Zhinong Zhang & Paul Dupree
5. Measuring processivity
   Svein J. Horn, Morten Sørlie, Kjell M. Vårum, Priit Väljamäe and Vincent G.H. Eijsink
6. Distinguishing xyloglucanase activity in endo-b (1® 4) glucanases
   Jens M. Eklöf, Marcus C. Ruda and Harry Brumer
7. Methods for structural characterization of the products of cellulose- and xyloglucan-hydrolyzing enzymes
   Maria J. Peña, Sami T. Tuomivaara, Breeanna R. Urbanowicz, Malcolm A. O’Neill and William S. York
8. The crystallization and structural analysis of cellulases (and other glycoside hydrolases): strategies and tactics
   Shirley M. Roberts and Gideon J. Davies
9. Visualization of Cellobiohydrolase I from Trichoderma reesei Moving on Crystalline Cellulose Using High-Speed Atomic Force Microscopy
   Kiyohiko Igarashi, Takayuki Uchihashi, Anu Koivula, Masahisa Wada, Satoshi Kimura, Merja Penttilä, Toshio Ando and Masahiro Samejima
10. Small angle X-ray scattering and crystallography; a winning combination for exploring the multi-modular organisation of cellulolytic macromolecular complexes
    Mirjam Czjzek, Henri-Pierre Fierobe and Véronique Receveur-Bréchot
11. Quantitative approaches to the analysis of carbohydrate-binding module function
    D. Wade Abbott‡ and Alisdair B. Boraston
12. In Situ Detection Of Cellulose With Carbohydrate-Binding Modules
    J. Paul Knox
13. Interactions between family 3 carbohydrate binding modules (CBMs) and cellulosomal linker peptides
    Oren Yaniv. Felix Frolow. Maly Levy-Assraf, Raphael Lamed and Edward A. Bayer
14. Approaches for improving thermostability characteristics in cellulases
    Michael Anbar and Edward A. Bayer
15. Thermophilic glycosynthases for oligosaccharides synthesis
    Beatrice Cobucci-Ponzano, Giuseppe Perugino, Andrea Strazzulli, Mosè Rossi and Marco Moracci
16. Engineering cellulase activity into Clostridium acetobutylicum
    Henri-Pierre Fierobe, Florence Mingardon and Angélique Chanal
17. Transformation of Clostridium thermocellum by electroporation
    Daniel G. Olson and Lee R. Lynd
18. Genetic and Functional Genomic Approaches for the Study of Plant Cell Wall Degradation in Cellvibrio japonicus
    Jeffrey G. Gardner and David H. Keating
19. Methods for the isolation of cellulose-degrading microorganisms
    James E. McDonald, David J. Rooks and Alan J. McCarthy
20. Metagenomic approaches to the discovery of cellulases
    David J. Rooks, James E. McDonald and Alan J. McCarthy
21. Escherichia coli expression, purification, crystallization and structure determination of bacterial cohesin-dockerin complexes
    Joana Brás, Ana Luisa Carvalho, Aldino Viegas, Shabir Najmudin, Victor D. Alves, José A.M. Prates, Luís M.A. Ferreira, Maria J. Romão, Harry J. Gilbert and Carlos M.G.A. Fontes
22. Measurements of relative binding of cohesin and dockerin mutants using an advanced ELISA technique for high-affinity interactions
    Michal Slutzki, Yoav Barak, Dan Reshef, Ora Schueler-Furman, Raphael Lamed and Edward A. Bayer
23. Designer cellulosomes for enhanced hydrolysis of cellulosic substrates
    Yael Vazana, Sarah Moraïs, Yoav Barak, Raphael Lamed and Edward A. Bayer
24. High-throughput screening of cohesin mutant libraries on cellulose microarrays
    Michal Slutzki, Vered Ruimy, Ely Morag, Yoav Barak, Rachel Haimovitz, Raphael Lamed and Edward A. Bayer