Methods of Adipose Tissue Biology, Part A (Methods in Enzymology, Vol. 537)

Table of Contents

1. Adipocyte-Specific Transgenic and Knockout Models
Sona Kang, Xingxing Kong and Evan D. Rosen

2. Imaging White Adipose Tissue With Confocal Microscopy
Gabriel Martinez-Santibañez, Kae Won Cho and Carey N. Lumeng

3. Isolation and Study of Adipocyte Precursors
Christopher Church, Ryan Berry and Matthew S Rodeheffer

4. Imaging of Adipose Tissue
Ryan Berry, Christopher Church, Martin T. Gericke, Elise Jeffery, Laura Colman and Matthew S. Rodeheffer

5. Adipose Tissue Angiogenesis Assay
Raziel Rojas-Rodriguez, Olga Gealekman, Maxwell E. Kruse, Brittany Rosenthal, Kishore Rao, SoYun Min, Karl D. Bellve, Lawrence M. Lifshitz and Silvia Corvera

6. Quantifying Size and Number of Adipocytes in Adipose Tissue
Sebastian D. Parlee, Stephen I. Lentz, Hiroyuki Mori and Ormond A. MacDougald

7. Use of Osmium Tetroxide Staining with Micro-Computerized Tomography to Visualize and Quantify Bone Marrow Adipose Tissue in Vivo
Erica L. Scheller, Nancy Troiano, Joshua N. VanHoutan, Mary A. Bouxsein, Jackie, A. Fretz, Yougen Xi, Tracy Nelson, Griffin Katz, Ryan Berry, Chris D. Church, Casey R. Doucette, Matthew S. Rodeheffer, Ormond A. MacDougald, Clifford J. Rosen and Mark C. Horowitz

8. Brown Adipose Tissue in Humans: Detection and Functional Analysis Using PET (Positron Emission Tomography), MRI (Magnetic Resonance Imaging), and DECT (Dual Energy Computed Tomography)
M. Borga, A.Virtanen Kirsi, T.Romu, O.Dahlqvist Leinhard, A.Persson, P.Nuutila and S. Enerbäck

9. Analysing the Functions and Structure of the Human Lipodystrophy Protein Seipin
M.F. Michelle Sim, Md Mesbah Uddin Talukder, Rowena J. Dennis, J. Michael Edwardson and Justin J. Rochford

10. Differentiation of Human Pluripotent Stem Cells into Highly Functional Classical Brown Adipocytes
Miwako Nishio and Kumiko Saeki

11. Analysis and Measurement of the Sympathetic and Sensory Innervation of White and Brown Adipose Tissue
Cheryl H. Vaughan, Eleen Zarebidaki, J. Christopher Ehlen and Timothy J. Bartness

12. Measurement and manipulation of Human Adipose Tissue Blood Flow Using Xenon Washout Technique and Adipose Tissue Microinfusion.
Richard Sotornik and Jean-Luc Ardilouze

13. Isolation and Quantitation of Adiponectin Higher Order Complexes
Joseph M. Rutkowski and Philipp E. Scherer

14. Genome-Wide Profiling of Transcription Factor Binding and Epigenetic Marks in Adipocytes by ChIP-seq
Ronni Nielsen and Susanne Mandrup

15. Analysis and Isolation of Adipocytes by Flow Cytometry
Susan M. Majka, Heidi L. Miller, Karen M. Helm, Alistaire S. Acosta, Christine R. Childs, Raymond Kong and Dwight J. Klemm

16. Flow Cytometry Analyses of Adipose Tissue Macrophages
Kae Won Cho, David L. Morris and Carey N. Lumeng

Author Info

Ormond A Macdougald, John A. Faulkner Collegiate Professor in Physiology, and a Professor of Molecular & Integrative Physiology, and Internal Medicine, University of Michigan Medical School